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Brains were collected and stored in 4% PFA for 24 h after which they were transferred to 20% sucrose for cryoprotection at 4 C
Another limitation of the studies was the use of different comparators ranging from placebo controls to other medications for T2DM (including insulin), which have different effects on bone tissue and were included in only one group, misleading the interpretation
E.NicholsA
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