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Cytotoxicity assay Effector (NK) and target (fungal) cells were grown as described above

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At the end of the experiment (48 hours), 100 l were transferred to a cytometer tube, 0.5 l of 25 M Calcein-AM were added and, after 15 min of incubation at room temperature, 5 l of fluorescently labeled beads plus 195 l of PBS 1X were added and cells were analyzed by flow cytometry

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