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The only way to confirm their absence is through a Limulus Amebocyte Lysate (LAL) assay a specialized test that pharmaceutical manufacturers run on every production batch per USP Chapter 85 requirements

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In parallel, excessive Cu + generated in GSH-rich reductive environments can bind lipoylated mitochondrial TCA-cycle proteins such as DLAT, inducing aberrant protein aggregation and triggering cuproptosis across multiple cancer types 187,189,192-194