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Briefly, 500 ng DNase-treated total RNA were used as input for amplification and biotin labeling reactions (Illumina TotalPrep RNA Amplification Kit, Ambion) prior to hybridization of the resulting cRNAs onto Illumina HumanHT-12_v4_BeadChips, washing, Cy3-streptavidin staining and scanning according to the manufacturers instructions

Vertical bands involving underlying muscle (platysmal bands) respond less to topical treatment but may still show some improvement in overlying skin texture

doi:10.1249/01.mss.0000227314.85728.35 Enomoto A, Wempe MF, Tsuchida H, et al