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Nagendra, L., BG, H., Sharma, M., & Dutta, D

Viral proteins as well as host damage-associated molecular patterns, that accumulate following cellular stress during viral infection, were linked to TLR4 activation, with uncontrolled TLR4 activation being associated with severe disease (Olejnik et al., 2018)

Immunoblotting Tissue and cell lysates were extracted from cells using RIPA buffer with protease inhibitor (Thermo Fisher Scientific, MA, USA)

Astrocytes induce pyroptosis by internalizing -syn aggregates and processing pro-caspase-1 to cleave GSDMD, releasing IL-1/IL-18 [259]