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To verify the absence of bacteria, flies from two different germ-free vials were crushed in ~ 200uL of sterile dH2O in an aseptic environment, then plated on blood agar

Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA1 but not VLA-4 integrin mediated human T-cell adhesion

RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

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10.1007/s00441-017-2738-1 Cell Tissue Res