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The GAPDH substrate 1,3 bisphosphoglycerate was generated by incubating 13.5 mM 3-phosphoglycerate, 6 mM ATP with 0.012 U/l 3-phosphoglycerate phosphokinase in 100 mM Tris/HCl p H 8.0, 1 mM EDTA, 15 mM MgCl 2 for 20 to 30 min at RT
Initially, injections are given daily or every other day for a week, then weekly for 12 months, followed by monthly maintenance
ZuoLPratherERStetskivMGarrisonDEMeadeJRPeaceTIet alInflammaging and oxidative stress in human diseases: from molecular mechanisms to novel treatments
doi:10.1002/hep.510250310 Acknowledgments Michele Malaguarnera was supported by the International PhD program in Neuropharmacology of the University of Catania
Produces metabolic byproducts that can shift pH in either direction depending on the organism
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