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The levels of serum testosterone (T), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in rat serum were detected by using an enzyme-linked immunoassay (ELISA)

For protein extraction, each tissue was placed into a tube containing a lysis buffer consisting of 20 mM Tris HCl with pH 7.6, 0.5 M Sucrose, 0.15 M KCl, 20 mM DTT supplemented with 100 L/mL of a protease inhibitor cocktail (Sigma-Aldrich, MO, USA) in a 1:3 (v/v) ratio