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[14] [15] The administration of N-acetylcysteine (NAC), a cysteine prodrug, helps replenish intracellular GSH levels

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Consistent with our previous data which indicated a protective role of GSH under cystine-deprived conditions, cell death was most severe when both cystine and GSH were depleted in HepG2 cells (Fig

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The infected supernatants were collected and pulled, filtered through a 0.45-m cellulose acetate filter (Costar) and then centrifuged at 500 g for 5 min at 4 C